Benjamin J. Darien D.V.M., M.S.

Diplomat American College of Veterinary Internal Medicine, Associate Professor of Large Animal Internal Medicine, Department of Medical Sciences, University of Wisconsin

Dr. Benjamin Darien is a scholar, published author, and is well-known among equine enthusiasts. Currently, he shares his expertise at the University of Wisconsin, College of Veterinary Medicine in the Department of Medical Sciences. With over 20 years of experience, Dr. Darien is well-versed in animal care and health.

Dr. Darien is Board Certified in Veterinary Internal Medicine and has specialized in many veterinary subjects, including Large Animal Diagnostics and Therapeutics, Hemostasis, and Veterinary Internal Medicine. With experience in private veterinary practice, large animal medicine and surgery, and scholastic courses, he has more than enough skill to care for horses under every circumstance.

A long list of published works is credited to Dr. Darien. His papers can be found in scientific and research publications. Dr. Darien has presented his writings in the United States, England, France, Italy and many other countries around the world.  In addition to his relationship with TNI, Dr. Darien is associated with the American Heart Association, the International Society on Thrombosis and Hemostasis, the American College of Veterinary Medical Association, American Association of Equine Practitioners, the International Endotoxin Society, the American Thoracic Society, the Shock Society, and the European Shock Society.

“As a large animal internist, I have provided critical care to horses and foals for over 20 years. Today, horse owners can expect their horses and foals to live longer and perform better than ever before. Similarly, sick horses and foals have a greater chance of responding to medical therapy and returning to full function. Attention to nutritional supportive care is one of the reasons for these expectations.

“TAHITIAN NONI™ Equine Essentials™ is a product that directly addresses the health needs of the horses. This product is unique because its principle ingredients, the juice and oil of the noni plant, are well recognized for their potent antioxidant activities. Today, more than anytime in the past, professional and novice horse owners can effectively and economically provide good quality health care to their horses.”

Education
1982-1984  University of Tennessee             D.V.M., Veterinary Medicine
                    College of Veterinary Medicine
                    Knoxville, Tennessee
1976-1979  University of California              M.S., Animal Science
                    Davis, College of Agriculture
                   & Environmental Sciences        
1973-1975  University of California Davis     B.S., Animal Science
                   College of Agriculture
                   & Environmental Sciences

Honors
2004    Resident Research Awards Presented at 2003 ACVIM Forum to Dr. Jo Ann Slack University of Wisconsin Biochemical Evidence of Myocardial Injury in Septic Foals

2003        Santa Cruz Biotechnology Investigator Award for "Equine platelet CD62P (P-selectin) expression: a phenotypic and morphologic study" published in Veterinary Immunology and Immunopathology

2001        Morris Animal Foundation Veterinary Honor Roll, an award paying tribute for the care and concern afforded to a particular patient.

1993        Scanning Microscopy: International Presidential Award, an award for innovative and significant contribution: Vascular casting of the equine colon: Application to an ischemia/reperfusion model.

1984        Upjohn Award for Clinical Proficiency in the Large Animal Clinic. Upjohn Co.

1978    Rotary International: Group Study Exchange with Japan.

Professional Experience
7/2003-present   Faculty Senator, Department of Medical Sciences, University of Wisconsin, Madison, WI
4/98-present       Associate Professor, Department of Medical Sciences, University of Wisconsin, Madison, WI
5/94-present       Full Affiliate Status, Assistant Professor, Department of Animal Health and Biomedical Sciences, University of Wisconsin, Madison, WI
9/93-present       Director, Comparative Hemostasis Laboratory, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI
7/89-9/91           Assistant Professor, College of Veterinary Medicine, Oregon State University, Corvallis, OR
7/88-6/89           Instructor of Equine Medicine, Department of Large Animal Clinical Sciences, Veterinary Clinical Center, Michigan State University, East Lansing, MI
2/89-3/89           Visiting professor, Atlantic Veterinary College, University of Prince Edward Island, Department of Health Management, Canada
7/86-6/88           Resident, Equine Medicine, Department of Large Animal Clinical Sciences, Veterinary Clinical Center, Michigan State University, East Lansing, MI
7/85-6/86           Internship, Large Animal Medicine and Surgery, College of Veterinary Medicine, Department of Large Animal Medicine, University of Georgia, Athens, GA
6/84-6/85           Private Practice, Daniel V. Flynn, Georgetown Equine Hospital, Georgetown Road, Charlottesville, VA

Publications
1. Risberg ÅI, Webb CB, Cooley AJ, Peek SF, Darien BJ.  Staphylococcus intermedius associated pastern leukocytoclastic vasculitis in a horse and a review of pastern dermatitides in horses.  Vet Record, 156:740-743, 2005.

2. Xu J, Lasry JB, Svaren J, Wagner B, Darien BJ.  Identification of Equine P-Selectin Glycoprotein Ligand-1 (CD162).  Mammalian Genome, 16:66-71, 2005.

3. Risberg ÅI, Slack JA, Semrad S, Peek SF, Darien BJ, McGuirk S. ECG of the month, JAVMA, 226:527-529, 2005.

4. Kuckleburg CJ, Inzana TJ, Corbeil LB, Darien, BJ, Czuprynski CJ.  Bovine platelets activated by Haemophilus somnus and its LOS induced apoptosis in bovine endothelial cells.  Microbial Pathogenesis, 38:23-32, 2005.

5. Bauer S, Livesey MA, Bjorling DE, Darien BJ.  Computed tomography assisted surgical correction of persistent right aortic arch in a neonatal foal.  Equine Vet Education, In Press.

6. Slack JA, McGuirk SM, Erb HN, Lien L, Coombs D, Semrad S, Risberg A, Marques F, Darien BJ, Fallon L, Burns P, Murakami A, Apple FS, Peek SF.  Biochemical Markers of Cardiac Injury in Normal, Surviving Septic, or Nonsurviving Septic Neonatal Foals.  J Vet Int Med. 19:577-580, 2005.

7. Peek SF, Borah S, Semrad S, McGuirk S, Slack JA, Patton E, Coombs D, Lien L, Darien BJ.  Detection of Plasma Endotoxin Concentrations in Equine Subjects: A Methods Study.  Vet Clin Path, 33:29-31, 2004.

8. Lalko CC, Deppe E, Ulatowski D, Lutgen A, Hart, AP, Patton EA, Lunn DP, Suresh M, Darien BJ.  Equine platelet P-selectin expression: A phenotypic and morphological study.  Vet Immunol Immunopath. 91:119-134, 2003.

9. Landolt G, Nemke B, Darien BJ. Kruse-Elliott KT.  Effect of inhaled endotoxin on cardiopulmonary function and E-selectin expression in pigs.  Am J Vet Res, 63:1302-1308, 2002

10. Garcia-Cantu MC, Hartmann FA, Brown CM, Darien BJ.  Bordetella bronchiseptica and equine respiratory infections: a review of 30 cases.  Equine vet Education, 12:45-50, 2000.

11. Robinson TM, Manley PA, Sims PA, Albrecht R, Darien BJ.  Cytokine and eicosanoid response by cultured human monocytes exposed to titanium particulate debris. Microscopy and Microanalysis, 5:344-351, 1999.

12. Forrest LJ, Cooley AJ, Darien BJ.  Digital arterial thrombosis in a septicemic foal. J Vet Int Med, 13:382-385, 1999.

13. Wilke VL, Darien BJ.  Acute respiratory distress in a pony.  Veterinary Forum, 15:74-76, 1998.

14. Darien BJ, Sims P, Robinson T, Manley P, Albrecht R.  Characterization of titanium alloy particulates to study the in vitro release of inflammatory mediators by human peripheral blood monocytes.  Microscopy and Microanalysis, 14(Supp.2):940-941, 1998.

15. McAnulty JF, Stone WC, Darien BJ.  The effects of ischemia and reperfusion on mucosal respiratory function, adenosine triphosphate, electrolyte, and water content in the ascending colon of ponies. Vet Surg, 26:172-181, 1997.

16. Darien BJ, Stone WC, Hart AP, Schilly DR, Clayton MK.  Systemic hemostatic alterations during no-flow ischemia and reperfusion of the ascending colon in ponies.  Comp Haematol Int, 6:63-60, 1996.

17. Nelson KM, Darien BJ, Konkle DM, Hartman FA.  Actinobacillus suis septicaemia in two foals.  Vet Record, 138:39-40, 1996.

18. Triplett EA, O'Brien RT, Wilson DG, Steinberg H, Darien BJ.  Thrombosis of the brachial artery in a foal.  J Vet Int Med, 10:330-332, 1996.

19. Darien BJ, Stone WC, Dubielzig RR, Clayton MK.  Morphologic changes of the ascending colon during experimental ischemia and reperfusion in ponies.  Vet Path, 32:280-288, 1995.

20. Darien BJ. Eosinophilic pneumonitis in foals and horses.  Comp Contin Educ Pract Vet, 16:1210-1212, 1994.

21. Darien BJ, Sims PA, Stone WC, Schilly DA, Dubielzig RR, Albrecht RM.  Ischemia/reperfusion injury of the ascending colon in ponies: A correlative study utilizing microvascular histopathology and corrosion casting.  Scanning Microsc, 4:1311-1320, 1993.

Abstracts
Expression and Function of the Purinergic Receptor P2X7 in Primary Human Monocytes.  Proceedings, 1st Int Workshop on Nucleotides and their Receptors in the Immune System. p 2, 2000.

Immunomodulation of events in sepsis:  Role of P-selectin/P-selectin ligand system.  Proceedings, Havemeyer Foundation Workshop: Equine Immunology in 2001, p 91-93.

The role of P-selectin in ischemia reperfusion injury: Does it regulate platelet-leukocyte aggregation in the horse?  Proceedings, 19th Annual Veterinary Medical Forum, ACVIM, p192-194, 2001.

LPS-induced platelet-monocyte aggregates results in P-selectin-PSGL-1 dependent signal transduction.  Proceedings, 2nd Annual Cell Signaling & growth Control Symposium, Abstract 2.

LPS-induced platelet-monocyte aggregates results in P-selectin-PSGL-1 dependent signal transduction.  Proceedings, 24th Annual Conf on Shock, Shock 15: Supplement 1, 14, 2001.

Platelet P-selectin-mediated inflammation & thrombosis during neonatal septicemia.  Proceedings, Havemeyer Foundation Workshop: Neonatal Ssepticemia Workshop 3 2001, p 31-34.

Activated platelets induce P-selectin/PSGL-1 dependent P2X7 receptor activation.  Proceedings, XVIII Congress International Soc Thrombosis Haemostasis, Supplement to the journal Thrombosis and Haemostasis, July 2001 Abstract: P2639.

rPSGL1-Ig regulates platelet mediated p-selectin psgl-1 dependent signaling and chemokine release in whole blood.  Proceedings, XVIII Congress International Soc Thrombosis Haemostasis, Supplement to the journal Thrombosis and Haemostasis, July 2001 Abstract: P1172.

Activated platelets induce P-selectin dependent P2X7 receptor stimulation in monocytes.  Keystone Symposia Molecular mechanisms of leukocyte trafficking, April 2002, abstract 105, p 99.

P2X7-dependent IL-1b expression in monocytes: The role of activated platelets.  6th Int Symposium Global Risk Coronary Heart Disease and Stroke: Assessment, Prevention, and Treatment.  June 2002, abstract p. 25.

Detection of plasma endotoxin concentrations from equine clinical patients: A methods study.  J Vet Int Med 2003;17:453.

Biochemical evidence of myocardial injury in septic foals.  J Vet Int Med 2003;17:387-88.

Effects of anti-endotoxin antibodies on survival & mods in septicemic foals. J Vet Int Med ACVIM 2004, abstr.

A prospective study of neonatal septicemia and factors influencing survival. Proceedings of the 50th Annual Convention of the American Association of Equine Practitioners, Denver, Colorado, December 2004, pp60-63.

Biochemical markers of cardiac injury in normal and surviving versus non-surviving septicemic neonatal foals. Journal of Veterinary Emergency and Critical Care, 14, Suppl 1, S15, 2004.

Book Chapters
1. Brown CM, Darien BJ.  Anemia-pale mucous membranes.  In: Problems in Equine Medicine. CM Brown Ed, Lea and Febiger, Philadelphia, PA.  1989, p. 161-176.

2. Darien BJ.  Duodenum.  In: Equine Endoscopy.  CM Brown, JL Traub Dargate, Eds, CV Mosby Co, Malvern PA.  1990, p. 139-144.

3. Wagner PC, Watrous BJ, Darien BJ.  Septic arthritis and osteomyelitis.  In: Current Therapy in Equine Medicine, 3rd ed. NE Robinson, Ed.  WB Saunders Co, Philadelphia, PA.  1991, p. 455-462.

4. Darien BJ, Feldman BF.  Hemostasis in the newborn foal.  In: Current Therapy in Equine Medicine 3rd ed. NE Robinson, Ed.  WB Saunders Co, Philadelphia, PA. 1991, p. 427-432.

5. Darien BJ.  Foal diseases.  Consulting editor.  In: Current Therapy in Equine Medicine 3rd ed. NE Robinson, Ed.  WB Saunders Co., Philadelphia, PA. 1991, p. 411-485.

6. Darien BJ.  Medical diseases: Horses.  In: Review Questions and Answers for Veterinary Boards: Large Animal Medicine & Surgery.  PW Pratt, Ed.  Am Vet Pub Inc., Goleta, CA. 1993, p. 14-82.

7. Darien BJ.  Medical diseases: Horses.  In: Review Questions and Answers for Veterinary Boards: Large Animal Medicine & Surgery, 2nd ed.  PW Pratt, Ed. Mosby-Year Book, Inc., St. Louis, Mo. 1998, p. 3-40.

8. Darien BJ.  Section VIII Hemostasis: Chapter 78 Fibrinolysis.  In: Schalm’s Veterinary Hematology 5th Ed.  B Feldman, J Zinkl, NC Jain, Eds.  WB Saunders Co, Philadelphia, PA.  p 544-548, 2000.

9. Darien BJ.  Section VIII Hemostasis: Chapter 85: Acquired Coagulopathy V: Thrombosis.  In: Schalm’s Veterinary Hematology 5th Ed.  B Feldman, J Zinkl, NC Jain, Eds.  WB Saunders Co, Philadelphia, PA.  p 574-580, 2000.

10. Darien BJ and Risberg, A.  Thrombosis, Embolism, and Aneurysm.   In The Merck Veterinary Manual 9th Ed. Pp 109-113.

11. Darien B.J.  Disseminated intravascular Coagulation. 5 Minute Veterinary Consult; Ruminants, 1st edition, Lippincott Williams and Wilkins, Philadelphia, Ed Scott R. Haskell. In press.

12. Darien B.J.  Circulatory Shock. 5 Minute Veterinary Consult; Ruminants, 1st edition, Lippincott Williams and Wilkins, Philadelphia, Ed Scott R. Haskell. In press.

Oral Presentations
Effects of anti-endotoxin antibodies on survival & MODS in septicemic foals.  22nd Annual ACVIM Forum Minneapolis, Minnesota, 2004.
Biochemical markers of cardiac injury in normal and surviving versus non-surviving septicemic neonatal foals.  10th International Veterinary Emergency and Critical Care Symposium, San Diego, California, 2004.
A prospective study of neonatal septicemia and factors influencing survival.  50th Annual Convention of the American Association of Equine Practitioners, Denver, Colorado, 2004
Biochemical evidence of myocardial injury in septic foals.  21st Annual ACVIM Forum Charlotte, North Carolina, 2003.
Detection of plasma endotoxin concentrations from equine clinical patients: A methods study.  21st Annual ACVIM Forum Charlotte, North Carolina, 2003.
P2X7-dependent IL-1b expression in monocytes: The role of activated platelets.  6th Int Symposium Global Risk Coronary Heart Disease and Stroke: Assessment, Prevention, and Treatment.  Florence, Italy, 202.
Activated platelets induce P-selectin dependent P2X7 receptor stimulation in monocytes.  Keystone Symposia: Molecular mechanisms of leukocyte trafficking, Steamboat Springs, CO, 2002.
Platelet P-selectin-mediated inflammation & thrombosis during neonatal septicemia.  Havemeyer Foundation Workshop 3: Neonatal Septicemia, Talloires, France, invited speaker, 2001.
rPSGL1-Ig regulates platelet mediated P-selectin PSGL-1 dependent signaling and chemokine release in whole blood.  XVIII Congress International Soc Thrombosis Haemostasis, Paris France, 2001.
Activated platelets induce P-selectin/PSGL-1 dependent P2X7 receptor activation.  XVIII Congress International Soc Thrombosis Haemostasis, Paris France, 2001.
The role of P-selectin in ischemia reperfusion injury: Does it regulate platelet-leukocyte aggregation in the horse?  19th Annual Veterinary Medical Forum, ACVIM, Denver, CO, 2001.
Immunomodulation of events in sepsis: Role of P-selectin/P-selectin ligand system.  Havemeyer Foundation Workshop: Equine Immunology in 2001, Santa Fe, NM, invited speaker, 2001.

Research Grants

• Equine PSGL1-Ig regulation of inflammation and thrombosis.
• Platelet-leukocyte aggregate formation during intestinal ischemia and inflammatory bowel disease.
• Platelet activation during equine intestinal ischemia and inflammatory bowel disease.
• The P-selectin/P-selectin ligand system:  PCR amplification and sequence analysis of equine P-selectin glycoprotein ligand.
• Effects of anti-endotoxin antibodies on survival and multiple organ dysfunction in septicemic neonatal foals.
• The P-selectin/P-selectin ligand system in the horse: Proof of principle that it regulates neutrophils adhesion.
• Purinergic receptor regulation of signal transduction pathways and cellular activation in P-selectin adhered monocytes.
• Priming and pathologic changes in the respiratory tract of pigs following exposure to feed, endotoxin and b-1,3-glucan.
• Understanding inflammation in the pig lung: What role does influenza virus play.
• Priming mechanisms in porcine lung disease.
• The role of P-selectin in priming inflammatory cells.
• Cardiopulmonary effects of low molecular weight heparin and PAF antagonist during endotoxemia.